MODULATORS :: APPLICATIONS :: Measurement of Degree of Polarization of Fluorescence/Luminescence

The polarization of fluorescence radiation emitted by a sample is frequently correlated with the (linear) polarization of the exciting radiation.  Usually the emitted light polarization is parallel to the exciting light polarization.  A parameter P (Equation 1) defines the degree of polarization of fluorescent light.

EXPERIMENTAL SETUP



             

Where I_|| = the intensity of fluorescence light parallel to excitation,     I= Intensity of fluorescent light normal to excitation, A₀ = /2 (/4 waves)  and J₁(A₀) = 0.567

ADDITIONAL TOOLS
Fluorescence Complete Solution Packages


COMPANIES USING PEMS TO MEASURE FLUORESCENCE POLARIZATION:
On-Line Instrument Systems


ADDITIONAL READING
Recording Polarization of Fluorescence Spectrometer - A Unique Application of Piezoelectric Birefringence Modulation, John E. Wampler and Richard J. DeSa, Analytical Chemistry, 46, 563 (1974)

K.W. Hipps and G.A. Crosby, Applications of the Photoelastic Modulator to Polarization Spectroscopy, J. Phys. Chem 83, 555-562 (1979).