Technology for Polarization Measurement

Exicor® MicroImagerTM

The Hinds Birefringence Imaging Microscope is ideal for measuring birefringence in biological structures (see Figure 1 below), glass, crystals, and many other organic and inorganic samples.

Features:


• No Dyes or Fluorescent labels required
• Measures retardation, angle and intensity
• Images retardation, angle and intensity
• Allows color maps to be customized by user for optimal display of data
• Allows maximum, average, and standard deviation over entire image and in user-selected areas or lines
• View data by intensity, birefringence/retardation, angle, or combination
• Histograms for statistical analysis
• User calibration
• Export of data in .csv and binary formats
• Export of data from user selected areas
• Export images in bit, png or tiff formats
• Compatability with third party analysis tool, ImageJ

Phase unwrapping technology is now included with every MicroImager.

→ For highly birefringent samples, the Exicor® MicroImagerTM has the ability to measure up to 3500nm with 4 color configuration and up to 2400nm with 3 color configuration.

 

 

2X Mouse Brain jet rev1-24-2017

Plastic sheet with ding_2X

 

Figures 1 & 2. Birefringence images, left to right: myelinated white matter in a mouse brain; damaged plastic sheet.
Diamond Sample MicroImager
Acrylic bond with optic

 

Figures 3 & 4. Birefringence images, left to right: stress measured in diamond sample; acrylic bonded to an optic.

 

SPECIFICATIONS

Retardation Repeatability ≤ 0.6 nm (3 sigma)
Light Source Wavelengths Red (655 nm)
Amber (615 nm)
Green (530 nm)
Blue (475 nm)
Retardation Measurement Range with single color 1 nm - 0.5λ nm
Retardation Measurement Range with Phase Unwrapping 3 Color: 1 nm - 2400 nm
4 Color: 1 nm - 3500 nm
Measurement Speed with Single Color Nominal 7 sec
Dimensions Footprint 30.5 cm x 25.4 cm
Height 68.5 cm
Stage Travel 75 mm, 56 mm

 

RESOLUTION AND FIELD OF VIEW

 

 Imager Resolution 2048 nm x 2048 nm
Bit-Depth 12 bits
 Objective Resolution Field of View
2X  5 micron  5.6 mm x 5.6 mm
5X  2 micron  2.2 mm x 2.2 mm
10X  1 micron  1 mm x 1 mm

 

FURTHER READING:

J. J. Cardiel, Y. Zhao, P. de la Iglesia, L. D. Pozzo and A. Q. Shen, Soft Matter, 2014, DOI: 10.1039/C4SM01920B.

 
In the mid-2000's, Hinds Instruments entered into an agreement with Cambridge Research and Instrumentation (CRi) to represent the LCPolscope® product line and later the Abrio® product line. After a series of business transitions, the Abrio product line was discontinued by Perkin Elmer. At the same time, Hinds Instruments introduced the Exicor® MicroImagerTM, which improved on CRi's liquid crystal technology using the Hinds Photoelastic Modulator (PEM) technology.

 
Contact us for more information about the Exicor® MicroImagerTM.